Part:BBa_K4404003

acsD, acsC and acsE from A. woodii

This is a fragment with a length of 3.445 kb, which was synthesized by de-novo-synthesis with the genomic background of Acetobacerium woodii. It consists of the genes ascD, acsC and acsE. acsD and acsC code for the acetyl-CoA synthase subunit (ACS) of the CODH/ACS-complex which transfers methyl-group from Co-FeS-P to Ni-Fe complex. acsE codes for the methyltransferase subunit which transfers the methyl group generated from CO2 to Co-FeS-P. All these genes are parts of the Wood-Ljungdahl pathway of acetogenic bacteria. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a SalI restriction site and a 5 base pairs overhang. Downstream it is flanked by a SfaAI restriction site and a 5 base pairs overhang.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2405
  Illegal PstI site found at 142
  Illegal PstI site found at 1577
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2405
  Illegal PstI site found at 142
  Illegal PstI site found at 1577
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2405
  Illegal XhoI site found at 2320
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2405
  Illegal PstI site found at 142
  Illegal PstI site found at 1577
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2405
  Illegal PstI site found at 142
  Illegal PstI site found at 1577
  Illegal NgoMIV site found at 2017
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2291